Amino Acids, Peptides and Proteins: An Introduction by H.D. Jakubke, J. Jeschkeit

By H.D. Jakubke, J. Jeschkeit

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They are based on the estimation of the products formed on treatment of amino acids with specific enzymes. 5 mg amino acid can be detected. Table 11 shows the most important enzymatic processes used in amino acid determination. Table 11. 6. Microbiological Processes66 Several microorganisms-especially lactic acid bacteria-may be used for qualitative and quantitative analyses of amino acid which depend on their different amino acid requirements. 5* 52 Amino Acids The amino acid to be estimated is incorporated into a growth medium alone with all the other requirements for normal growth of the microorganism.

With the increasing demand for (for example) glutamic acid, glycine, alanine and aspartic acid for use as flavouring agents in the food industry, of lysine, methionine, threonine, tryptophan and other essential amino acids as additives for nutritionally inferior foodstuffs and of serine, threonine and cysteine as intermediates in the cosmetic industry, the production of amino acids is now performed on an industrial scale. In the 1950s glutamic acid was isolated exclusively from corn proteins and sweet turnip molasses by hydrolysis.

GARAY in 1968. He used the /]-radiation of 90Sr as a model for circularly polarised light and found that after exposure of DL-tyrosine to this radiation for 18 months, destruction of the D-antipode was favoured over destruction of the L-antipode. Other possibilities include differential solubility of the optical antipodes and differentiation between isomers at optically active inorganic surfaces such as quartz. Once optical activity is generated it can be transferred spontaneously into other DL-compounds.

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